BioSAXS is a popular technique for obtaining structural information from biomolecules in solution:
- determining physiological oligomeric state
- validating proposed models of complexes
- building complexes from monomers or known fragments
- studying protein-protein interactions under extreme conditions
- refining pseudoatomic models
- categorizing discrete folded and unfolded states
- finding volume fractions in mixtures
- calculating molecular volume, radius of gyration, diameter
- quantifying changes in flexibility
- measuring pair distance distribution functions
- computing low-resolution electron density
We can provide BioSAXS data routinely on samples at up to 400 MPa (4 kbar). Measurements at up to 600 MPa are available upon special request. Many protein samples are not monodisperse, but rather consist of mixtures of oligomers in equilibrium that must be separated for analysis. We are the only facility in the world that can also perform size-exclusion and ion-exchange chromatographic separations at up to 100 MPa while performing simultaneous BioSAXS measurements.
How to Obtain Access
The HP-Bio/BioSAXS facility runs 24 hours a day, 6 days a week for three 8-week periods during the year. Synchrotron beamtime is available free of charge to qualified researchers worldwide doing non-proprietary work through a standard online proposal mechanism.
Richard Gillilan Cornell University, USA